Cryptosporidium felis Infection, Spain

Comparison of community-and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA. Staphylococcus aureus isolated in cases of impetigo produces both epidermolysin A or B and LukE-LukD in 78% of 131 retrospective and prospective cases. Lerakis S. Staphylococcus aureus bac-teremia and endocarditis: the Grady Memorial Hospital experience with methi-cillin-sensitive S. aureus and methicillin-resistant S. aureus bacteremia. RA. Development of a clinical data warehouse for hospital infection control. To the Editor: Coccidian proto-zoans that belong to the genus Cryptosporidium frequently cause gastrointestinal infection in humans and animals and are distributed worldwide. Cryptosporidium hominis and the cattle genotype of C. parvum are responsible for most human infections. However, other species and genotypes of Cryptosporidium, such as C. felis, C. muris, C. meleagridis, C. canis, C. parvum pig genotype, and C. parvum cervine genotype, have also been detected in stool samples of immunosuppressed and immunocom-petent patients (1). Since 1999, when Pieniazek et al. described 3 cases of C. felis infection in HIV-positive patients (2), several studies have confirmed that this species can infect humans. Recently, Muthsusamy et al. described C. felis infections in 5 HIV-positive persons in southern India (3). In this article, we describe our experience with an imported case of C. felis infection in Spain. A pediatrician requested a para-sitologic study for an immunocompe-tent, 4-year-old boy with diarrhea. The child came from an orphanage in Calcutta, India; he had arrived in Spain 10 days earlier after having been adopted by a Spanish family. Stool specimens were tested for a wide panel of enteric pathogens, including bacteria, viruses, and parasites. Cryptosporidium oocysts were detected by direct microscopic visual-ization of the samples, which had been concentrated by formalin–ethyl acetate sedimentation and stained with a modified Ziehl-Neelsen stain. Results were also positive for Cryptosporidium for samples tested by using an immunochromatographic DNA was extracted as described elsewhere (5), purified with polyvinyl-pyrrolidone, and stored at –20°C in Tris-EDTA buffer. After DNA extraction, PCR–restriction fragment length polymorphism (RFLP) analysis was performed by using previously described protocols based on the small subunit (SSU) rRNA gene (6), with digestion of the amplicon by the restriction enzymes SspI for species diagnosis or VspI for C. parvum genotype identification. For DNA sequencing, PCR products of the 18S rRNA gene fragments were purified and used for direct sequenc-ing in an ABI377 automated sequencer RFLP analysis showed a profile distinct from those of C. hominis and C. parvum cattle genotype and consistent with the …